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Last Updated :2026/04/05

Ryota Tsuchihashi

Department of Phatmaceutics
Lecturer/Senior Assistant Professor

Researcher information

■ Degree
  • 博士(薬学), 熊本大学
  • Pharmaceutical Doctor, Kumamoto University
■ Research Keyword
  • hepato protective activity
  • estrogenic activity
  • nephroprotective activity
  • intestinal bacteria metabolism
  • isoflavone
  • natural medicies
■ Field Of Study
  • Life sciences, Pharmaceuticals - chemistry and drug development, Chemical Pharmaceutical Science

Career

■ Career
  • 01 Apr. 2020
    福岡大学 薬学部, 生薬学教室, 講師
  • 01 Apr. 2007
    福岡大学 薬学部, 生薬学教室, 助教
  • 01 Apr. 2003
    faculyt of pharmaceutical sciences, fukuoka university, 生薬学教室, 助手
■ Educational Background
  • 2003
    Kumamoto University, Graduate School, Division of Pharmaceutical Sciences
  • 2000
    Kumamoto University, Faculty of Pharmaceutical Science, 薬科学科

Research activity information

■ Award
  • 16 Sep. 2005
    日本生薬学会「Natural Medicines」誌 論文賞
■ Paper
  • Two New Triterpene Glycosides in the Roots of Uraria crinita
    KINJO Junei; OKAWA Masafumi
    Chem Pharm Bull, Feb. 2019, 67(2):159 - 162, Refereed
  • Seven new resin glycosides from the seeds of Quamoclit × multifida
    KINJO Junei; TSUCHIHASHI Ryota; OKAWA Masafumi
    J Nat Med, Jan. 2019, 72(3):11 - 22, Refereed
  • Role of vasopressin V1a receptor in ∆9-tetrahydrocannabinol-induced cataleptic immobilization in mice
    KINJO Junei; TSUCHIHASHI Ryota
    Psychopharmacology, 2017, 2017:3475 - 3483, Refereed
  • Screening of promising chemotherapeutic candidates from plants against human adult T-cell leukemia/lymphoma (IV): phenanthroindolizidine alkaloids from Tylophora tanakae leaves
    NAKANO Daisuke; TSUCHIHASHI Ryota; OKAWA Masafumi; KINJO Junei
    J Nat Med, 2015, 69(3):397 - 401, Refereed
  • Analysis of Seasonal Variation in the Amounts of Phytochemicals in Kudzu (Pueraria lobata) Leaves Using HPLC with Evaporative Light Scattering Detection
    TSUCHIHASHI Ryota; NAKANO Daisuke; OKAWA Masafumi; KINJO Junei
    International Journal of Plant Biology and Research, 2015, 3(1):397 - 401, Refereed
  • Screening of promising chemotherapeutic candidates from plants against human adult T-cell leukemia/lymphoma (III)
    KINJO Junei; NAKANO Daisuke; TSUCHIHASHI Ryota; OKAWA Masafumi
    J Nat Med, Sep. 2013, 67(4):894 - 903
  • Screening of chemotherapeutic candidates from plants against ATL (II): apoptosis of antiproliferactive principle (24,25-dihydrowithanolide D) and structure-activity relationships with withanolides isolated from solanaceous plants
    KINJO Junei; NAKANO Daisuke; OKAWA Masafumi; TSUCHIHASHI Ryota
    J Nat Med., Feb. 2013, 67(2):415 - 420, Refereed
  • 腸内細菌による配糖体の加水分解と代謝活性化
    KINJO Junei; TSUCHIHASHI Ryota
    腸内細菌学雑誌, Nov. 2012, 26(11):223 - 233, Refereed
  • Screening of promising chemotherapeutic candidates against human adult T-cell leukemia/lymphoma from plants: active principles from Physalis pruinosa and structure-activity relationships with withanolides.
    NAKANO Daisuke; ISHITSUKA KENJI; TSUCHIHASHI Ryota; OKAWA Masafumi; TAMURA Kazuo; KINJO Junei
    J Nat Med., Jul. 2011, 65(3-4):559 - 567, Refereed
  • Development of an enzyme-linked immunosorbent assay to determine puerarin and its aglycone daidzein
    B. Pongkitwitoon; S. Sakamoto; H. Tanaka; TSUCHIHASHI Ryota; KINJO Junei; S. Morimoto; W. Putalun
    J. Nat. Med., Feb. 2011, 65(1):31 - 36, Refereed
  • Expression, Purification, and Characterization of Anti-Plumbagin Single-Chain Variable Fragment Antibody in Sf9 Insect Cell
    Seiichi Sakamoto; Futoshi Taura; Ryota Tsuchihashi; Waraporn Putalun; Junei Kinjo; Hiroyuki Tanaka; Satoshi Morimoto
    HYBRIDOMA, Dec. 2010, 29(6):481 - 488, Refereed
  • Simultaneous analysis of isoflavones and saponins in Pueraria flowers using HPLC coupled to an evaporative light scattering detector and isolation of a new isoflavone diglucoside
    Niiho Y; Nakajima Y; Yamazaki T; Okamoto M; Tsuchihashi R; Kodera M; Kinjo J; Nohara T
    Journal of Natural Medicines, Jun. 2010, 64(3):313 - 320, Refereed
  • Enzyme-Linked Immunosorbent Assay for Total Isoflavonoids in Pueraria candollei Using Anti-Puerarin and Anti-Daidzin Polyclonal Antibodies
    Benyakan Pongkitwitoon; Seiichi Sakamoto; Hiroyuki Tanaka; Ryota Tsuchihashi; Junei Kinjo; Satoshi Morimoto; Waraporn Putalun
    PLANTA MEDICA, May 2010, 76(8):831 - 836, Refereed
  • Development of sensitivity-improved fluorescence-linked immunosorbent assay using a fluorescent single-domain antibody against the bioactive naphthoquinone, plumbagin
    Seiichi Sakamoto; Futoshi Taura; Benyakan Pongkitwitoon; Waraporn Putalun; Ryota Tsuchihashi; Junei Kinjo; Hiroyuki Tanaka; Satoshi Morimoto
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Apr. 2010, 396(8):2955 - 2963, Refereed
  • Delta(9)-tetrahydrocannabinol enhances an increase of plasma corticosterone levels induced by forced swim-stress
    Sano K; Koushi E; Irie K; Higuchi S; Tsuchihashi R; Kinjo J; Egashira N; Oishi R; Uchida N; Nagai H; Nishimura R; Tanaka H; Morimoto S; Mishima K; Iwasaki K; Fujiwara M
    Biol Pharm Bull., Dec. 2009, 32(12):2065 - 2067, Refereed
  • Three new diterpenoids from the fruit of Vitex agnus-castus
    Ono M; Nagasawa Y; Ikeda T; Tsuchihashi R; Okawa M; Kinjo J; Yoshimitsu H; Nohara T
    Chem Pharm Bull, Oct. 2009, 57(10):1132 - 1135
  • Microbial transformation and bioactivation of isoflavones from Pueraria flowers by human intestinal bacterial strains
    Ryota Tsuchihashi; Mitsuru Kodera; Seiichi Sakamoto; Yoshijiro Nakajima; Takashi Yamazaki; Yujiro Niiho; Toshihiro Nohara; Junei Kinjo
    JOURNAL OF NATURAL MEDICINES, Jul. 2009, 63(3):254 - 260, Refereed
  • Construction and expression of specificity-improved single-chain variable fragments against the bioactive naphthoquinone, plumbagin.
    Sakamoto S; Taura F; Putalun W; Pongkitwitoon B; Tsuchihashi R; Morimoto S; Kinjo J; Shoyama Y; Tanaka H
    Biol Pharm Bull., Mar. 2009, 32(3):434 - 439, Refereed
  • Microbial metabolism of soy isoflavones by human intestinal bacterial strains
    Ryota Tsuchihashi; Seiichi Sakamoto; Mitsuru Kodera; Toshihiro Nohara; Junei Kinjo
    Journal of Natural Medicines, Sep. 2008, 62(4):456 - 460, Refereed
  • Three New Monoterpenoids from the Fruit of Genipa americana
    M.Ono; N. Ishimatsu; C. Masuoka; H. Yoshimitsu; R. Tsuchihashi; M. Okawa; J. Kinjo; T. Ikeda; T. Nohara
    Chem. Pharm. Bull., Apr. 2007, 55(4):632 - 634, Refereed
  • Hepatoprotective Constituents in Plants 15. Protective Effects of Natural-Occurring Flavonoids and Miscellaneous Phenolic Compounds on an HepG2 Cell Cytotoxicity Assay
    J. Kinjo; M. Hitoshi; R. Tsuchihashi; Y. Korematsu; M. Miyakoshi; T. Murakami; D. Araho; K. Mizutani; T. Tanaka; G. Nonaka; T. Nohara; M. Okawa; H. Okabe
    Journal of Natural Medicines, Jan. 2006, 60(1):36 - 41, Refereed
  • Antiproliferative Constituents from Umbelliferae Plants VII. Active Triterpenes and Rosmarinic Acid from Centella asiatica
    Miyako YOSHIDA; Masahiro FUCHIGAMI; Tsuneatsu NAGAO; Hikaru OKABE; Kazuhisa MATSUNAGA; Jiro TAKATA; Yoshiharu KARUBE; Ryota TSUCHIHASHI; Junei KINJO; Kunihide MIHASHI; Toshihiro FUJIOKA
    Biol. Pharm. Bull., Jan. 2005, 28(1):173 - 175, Refereed
  • Antiproliferative constituents from umbelliferae plants VII. Active triterpenes and rosmarinic acid from Centella asiatica
    Miyako Yoshida; Masahiro Fuchigami; Tsuneatsu Nagao; Hikaru Okabe; Kazuhisa Matsunaga; Jiro Takata; Yoshiharu Karube; Ryota Tsuchihashi; Junei Kinjo; Kunihide Mihashi; Toshihiro Fujioka
    Biological and Pharmaceutical Bulletin, 2005, 28(1):173 - 175, Refereed
  • Soy Isoflavone Metabolites Isolated from Human Urine
    TSUCHIHASHI Ryota; KINJO Junei
    Natural Medicines, Apr. 2004, 58(2):71 - 75, Refereed
  • Interactions of Phytoestrogens with Estrogen Receptors a and b (III). Estrogenic Activities of Soy Isoflavone Aglycones and Their Metabolites Isolated from Human Urine
    KINJO Junei; TSUCHIHASHI Ryota
    Biol. Pharm. Bull., Feb. 2004, 27(2):185 - 188, Refereed
  • HPLC Profile Analysis of Hepatoprotective Oleanene-Glucuronides in Several Soybean Processed Foods
    TSUCHIHASHI Ryota; KINJO Junei
    Natural Medicines, Dec. 2003, 57(6):242 - 245, Refereed
  • Hepatoprotective Constituents in Plants 14. Effects of Soyasapogenol B, Sophoradiol, and Their Glucuronides on the Cytotoxicity of tert-Butyl Hydroperoxide to HepG2 Cells
    KINJO Junei; TSUCHIHASHI Ryota; OKAWA Masafumi
    Biol. Pharm. Bull., Sep. 2003, 26(9):1357 - 1360, Refereed
  • Hepatopritective Constituents in Plants 14. Effects of Soyasapogenol B, Sophoradiol, and Thei Glucuronodes on the Cytotoxicity of tert- Butyl Hydroperoxide to HepG2 Cells. J.Kinho, T. Hirakawa, R. Tsuchihashi, T. Nagao. M. Okawa, T.Nohara, H. Okabe,
    Bio. Pharm. Bull., 2003, 26(9):1357 - 1360
  • Five triterpene glycosides from Oxytropis myriophylla
    M Okawa; R Yamaguchi; H Delger; R Tsuchihashi; T Nohara; J Kinjo; S Isoda; Y Ida
    CHEMICAL & PHARMACEUTICAL BULLETIN, Aug. 2002, 50(8):1097 - 1099, Refereed
  • Five Triterpene Glycosides from Oxytropis myriophylla M. Okawa, R. Yamaguchi, H. Delger, R. Tsuchihashi, T. Nohara, J. Kinjo, S. Isoda, Y. Ida,
    Chem. Pharm. Bull., 2002, 50(8):1097 - 1099
■ Books and other publications
  • 食用豆および大豆加工食品中の活性成分
    KINJO Junei; TSUCHIHASHI Ryota, Joint work, 226-238
    薬用食品の開発-薬用・有用植物の機能性食品素材への応用-、シーエムシー出版, Jan. 2007
■ Lectures, oral presentations, etc.
  • 腸内細菌による代謝研究(16)ヒト腸内細菌によるクロシンの代謝研究
    日本生薬学会第66回年会(東京都), Sep. 2019
  • 腸内細菌による代謝研究 (15) ヒト腸内細菌による淡竹葉フラボノイドの代謝研究
    日本薬学会第139年会 (千葉市), Mar. 2019
  • 大豆の生長段階における成分変化に関する研究―多変量解析法(主成分分析)をもちいて
    日本生薬学会第65回年会(広島市), Sep. 2018
  • ヒト腸内細菌株による代謝研究(14) -ヒト腸内細菌に よるクワの葉フラボノイドの代謝研究
    日本薬学会第138年会 (金沢市), Mar. 2018
  • ヒト腸内細菌株による代謝研究(13) ―Kaempferol配糖体の代謝研究―
    日本生薬学会第64回年会(船橋市), Sep. 2017
  • 葛花の新規イソフラボン配糖体に関する研究(2)
    Mar. 2017
  • 抱合化によるフィトケミカルの代謝活性化について
    KINJO Junei; TSUCHIHASHI Ryota
    平成22年度生理学研究所研究会 糖鎖機能研究会・・・分子レベルでの解明を目指して, 02 Jul. 2010
  • ヒト腸内細菌による代謝活性化研究 -「酒毒を解す」生薬・葛花に含まれるイソフラボンの肝保護作用について -
    土橋良太; 金城順英; 野原稔弘; 中島嘉次郎; 山崎 律; 新甫勇次郎
    第17回天然薬物の開発と応用シンポジウム, 14 Nov. 2008
  • Soy Isoflavone Metabolites from Human Urine
    TSUCHIHASHI Ryota; OKAWA Masafumi; KINJO Junei
    2006' International Symposium for Pharmaceutical in Beijing, 11 Oct. 2006
■ Affiliated academic society
  • The Japanese Society of Pharmacognosy
  • The Pharmaceutical Society of Japan
■ Industrial Property Rights
  • 特許5663576, NEURITE OUTGROWTH AGENT
    上西秀則, 金城順英, 土橋良太, 池田剛, 坂本昭宏